Cutoff to determine epidermal growth factor receptor (EGFR) gene amplification by fluorescence in situ hybridization (FISH) should be increased to >50% of EGFR-amplified (EGFR-amp) cells, and EGFR expression by real-time quantitative polymerase chain reaction (RT-qPCR) can act as a surrogate marker for EGFR-amp.
Why this matters
GBM is the most common and aggressive primary brain tumor, and EGFR is mutated in approximately half of all GBM tumors, leading to amplification of the gene.
Precision medicine specifically targeting EGFR-positive GBMs is under investigation, but there was no standard available for the method or cutoffs used to determine the amplification status of EGFR prior to this publication.
This publication should aid in the selection of patients into future precision medicine trials.
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